Thats happened a few time already (over the past few years). IIS likes to know who you are before it lets you talk to SQL server. So a nice blog here if you are connecting to a Sql Server db from IIS and the two machines are not under the same AD domain.
http://beyondthispoint.blogspot.com/2006/04/setting-up-iis6-application-pool.html
Do the steps, it works! This post is like an extended book mark!
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